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KMID : 0644120120250010020
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Journal of Oriental Gynecology
2012 Volume.25 No. 1 p.20 ~ p.33
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Effects of Kanghwalsokdan-tang on Dermal Fibroblast
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Yoo Jeong-Eun
Choi Kyung-Hee
Lim Hyun-Jung
Yoo Dong-Youl
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Abstract
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Objectives: This study was performed to elucidate the effects of Kanghwalsokdan-tang extract(KS) on hyper-plasy of collagen and cell damage in UVB-irradiated dermal fibroblast.
Methods: To demonstrate the effects of KS on wound healing we used human dermal fibroblast(F6). We evaluated the amount of increased PICP, TIMP-1 in dermal fibroblast. PICP, TIMP-1 concentration was measured using EIA kit. Also, we measured the nitrite production, and LDH release in UVB-irradiated dermal fibroblast to elucidate the action-mechanism of KS.
Results: 1. KS decreased the cell proliferation of dermal fibroblast. 2. KS decreased the biosynthesis of collagen in dermal fibroblast. 3. KS decreased the synthesis of TIMP-1 in dermal fibroblast. 4. KS had no effect on the LDH-release of UVB-irradiated dermal fibroblast. 5. KS inhibited nitrite production in UVB-irradiated dermal fibroblast.
Conclusions: From the results, we concluded that KS has a protective effect on wound healing and photoaging.
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KEYWORD
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Kanghwalsokdan-tang extract(KS), Dermal Fibroblast, UVB, Collagen, Wound healing, Photoaging
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